Skip to main content

The amygdala between sensation and affect: a role in pain

Abstract

The amygdala is a structure of the temporal lobe thought to be involved in assigning emotional significance to environmental information and triggering adapted physiological, behavioral and affective responses. A large body of literature in animals and human implicates the amygdala in fear. Pain having a strong affective and emotional dimension, the amygdala, especially its central nucleus (CeA), has also emerged in the last twenty years as key element of the pain matrix. The CeA receives multiple nociceptive information from the brainstem, as well as highly processed polymodal information from the thalamus and the cerebral cortex. It also possesses the connections that allow influencing most of the descending pain control systems as well as higher centers involved in emotional, affective and cognitive functions. Preclinical studies indicate that the integration of nociceptive inputs in the CeA only marginally contributes to sensory-discriminative components of pain, but rather contributes to associated behavior and affective responses. The CeA doesn’t have a major influence on responses to acute nociception in basal condition, but it induces hypoalgesia during aversive situation, such as stress or fear. On the contrary, during persistent pain states (inflammatory, visceral, neuropathic), a long-lasting functional plasticity of CeA activity contributes to an enhancement of the pain experience, including hyperalgesia, aversive behavioral reactions and affective anxiety-like states.

Background

Many cerebral structures, constituting the pain matrix, participate in nociception and in the expression and modulation of pain. They transmit and decode nociceptive information, generate, amplify or reduce the pain sensation, allow expression of defensive or distress behavior, and influence the affect. Among these brain structures, the amygdaloid complex, or amygdala, appears as a key component of the pain matrix [1]. Indeed, while this group of sub-cortical nuclei within the temporal lobe is well known for its critical role in the control of emotions [2], it has also been the target for a large number of fundamental research in the pain field during the two past decades. Overall, the amygdala is considered to provide an emotional value – either positive or negative – to sensory information, thus leading to adapted behavioral and affective responses and contributing to emotional memory. This role has been more particularly studied in the conditioned fear experimental paradigm [24]. Such conditioning is an adaptive process in response to potential danger. As nociceptive stimulations are also potentially harmful for body integrity, it is not surprising that the amygdala can integrate various nociceptive information to initiate or modulate the autonomic and behavioral responses according to the environmental context (internal and external) and to the affective state. This adaptation includes mechanisms of hypoalgesia/analgesia that allows reflex inhibition and facilitates fight or flight responses. Moreover, this brain structure participates in the anticipation/prediction of potential danger, based on salient sensory cues (sounds, images, odors…), to initiate the same adaptive responses as in direct presence of the danger. This is for example leading to conditioned or to stress-induced analgesia.

Pain includes a strong emotional component making it aversive and a reciprocal relation exists between pain and affective states. Stress can either inhibit or amplify pain; and anxiety as well as depressive states are often associated with more intense pain sensation, particularly when pain becomes chronic. In such chronic pain state, the amygdala could contribute to the hyperalgesia as well as to the anxio-depressive consequences of pain.

A few publications reviewed the role of the amygdala in persistent pain [5, 6]. The present review will consider the recent published data on the subject and address the key role of amygdala, especially its central nucleus, in various pain processes, either physiological or pathological. This will be done by reminding the anatomical context and presenting the morphological and functional evidences allowing insertion of the amygdala within the pain matrix. Then, we will show that the amygdala can exert either inhibitory or facilitating action on nociception and pain as well as on its affective component and consequences. Finally, we will address recent electrophysiological, neurochemical and biochemical data that are giving insights into underlying cellular and molecular mechanisms. While experimental data that are presented concern studies conducted in rodents, we will confront them with human brain imaging studies, thus stressing the potential role of the amygdala in human pain, especially with clinical relevance.

Review

Amygdala and extended amygdala

The amygdala is a nuclear complex with almond shape, located in the temporal lobe of mammals and identified by Burdach at the start of 19th century. It is an heterogeneous structure, grouping around a dozen of nuclei depending on the considered nomenclature, these nuclei being classically clustered in 4 groups: superficial, basolateral, central and medial (Figure 1) [7, 8]. The first two groups constitute the corticobasal amygdala that shows cortical-like characteristics [8]. Its main nuclei, the lateral and the basolateral nuclei are known as the basolateral amygdala (BLA) and mostly contain projection neurons of pyramidal type, synthesizing glutamate. It is reciprocally connected with the cerebral cortex, and sends a major projection to the striatal complex, especially in its ventral part [9, 10]. The BLA also densely innervates the central and medial groups of amygdala nuclei. These last two groups display an organization that is similar to the one of the basal ganglia, with striatopallidal-like neuronal morphology [8, 11]. Moreover, the central and the medial amygdala groups receive information from the cerebral cortex but do not send any direct projection to it. In addition to the four groups, a few nuclei of the amygdala remain unclassified, among them the intercalated cell masses which are small clusters of densely packed GABAergic neurons [12].

Figure 1
figure 1

Anatomical organization of the amygdala. A: Frontal section of a rat brain at the level of the amygdala processed for acetylcholinesterase staining. The boxed area indicates the position of the magnified region right-hand. The four main groups are outlined: superficial (purple), basolateral (red), central (blue), medial (green). B: Schematic representation of the main amygdala nuclei. The basolateral group (red) includes the lateral (L), basolateral (BL), basolateral ventral (BLV) and basomedial (BM) nuclei. The central group is represented by the central nucleus with its capsular (CeLC), lateral (CeL) and medial (CeM) subdivisions. The medial (green) and superficial (purple) groups are represented at this level by the medial (MeA) and anterior cortical (ACo) nuclei, respectively. The intercalated cell masses (ITC, black) appear wedged between BL and CeA. Other abbreviations: CPu: caudate-putamen; ic: internal capsule; GP: globus pallidus; ot: optic tract. Photographs of acetylcholinesterase staining from the authors’ archive.

The central nucleus of the amygdala (CeA), main component of the central group of amygdala nuclei, is considered as the output nuclei of the amygdaloid complex. It integrates information treated by the corticobasal group and it influences effector centers. The CeA is also reciprocally connected to more rostral forebrain structures, such as the lateral part of the bed nucleus of the stria terminalis (BSTL) and the dorsal part of the substantia innominata. This group of interconnected structures presents strong morphofunctional homogeneity and is designed as the central extended amygdala [8, 13]. In this review, we will more specifically detail data on the CeA, which is the most studied amygdaloid structure for its role in nociception and pain, and also refer to findings on the extended amygdala, as CeA functions are closely related to this forebrain macrostructure.

The CeA has three subdivisions: capsular (CeLC), central (CeL) and medial (CeM), that are defined based on cytoarchitecture, neurochemistry and connectivity [8, 11]. The CeLC and the CeL mostly contain medium-size spiny striatal-like neurons, while the CeM main neuronal population has a leptodendritic pallidal-like morphology (i.e. neurons with long dendrites with few or no spines). Neurons from these 3 sub-divisions co-synthesize GABA and neuropeptides, such as opioid peptides (enkephalins, dynorphin, endorphins), corticotropin-releasing factor (CRF), neurotensin, somatostatin, galanin or substance P [11, 1416]. These peptides and others, such as calcitonin gene-related peptide (CGRP), are also present in a dense neuropile of incoming axonal terminals [11]. Connections of the CeA (Figure 2) are characterized by strong relation, often reciprocal, with autonomic and modulatory centers of the hypothalamus and the brainstem. This includes the dorsal vagal complex, the parabrachial nucleus, the periaqueductal gray (PAG), the medullary and mesopontine reticular formation, the paraventricular nucleus of the hypothalamus and the lateral hypothalamus [7, 8, 11]. The midbrain dopaminergic centers, the locus coeruleus and the dorsal raphe are also under CeA control, and the CeA receives monoaminergic inputs from these structures. The connections of the CeA with the thalamus are sparser but reciprocal, and concern the paraventricular, posterior intralaminar and subparafascicular nuclei [8]. Cortical inputs mainly arise from non-isocortical areas (prefrontal, insular and perirhinal cortices) and from the corticobasal nuclei of the amygdala [11, 17]. While the CeA doesn’t directly project to the cerebral cortex, it has outputs toward substantia innominata cholinergic neurons that are projecting to the cortex [8]. The amygdaloid intercalated cell masses also provide an intra-amygdaloid input to the CeA [12]. Last, the CeA is interconnected with other components of the central extended amygdala through a dense network of reciprocal projections. Within the CeA, information follow a latero-median flux [11]: most afferents do synapses in the CeLC and the CeL, and neurons of these two subdivisions then influence the CeM where most of the efferent projection neurons of the CeA are found (Figure 2). Globally, these morphofunctional characteristics are similar to other components of the central extended amygdala [8]. This general organization of amygdala and extended amygdala described in rodent is similar to the human brain [8, 13].

Figure 2
figure 2

Main connections of the CeA involved in pain processes. The CeA received highly processed polymodal information from the cerebral cortex and the thalamus essentially through inputs from the lateral (L) and basolateral (BL) nuclei, and more direct nociceptive information from the parabrachial nucleus. Intercalated cell masses (ITC) provide an inhibitory input driven by L and BL. Most of these afferents contact neurons in the capsular (CeLC) and lateral (CeL) subdivisions of the central nucleus which control the activity of neurons in the medial subdivision (CeM). Efferents arise mainly from the CeM and target the other components of the central extended amygdala, thalamic nuclei and the integrative, modulator and effector centers in the hypothalamus and the brainstem. Most of these structures send a reciprocal projection to the central nucleus.

The CeA receives multiple nociceptive informations

Among the various CeA afferents, two main pathways are preferentially conveying nociceptive information (Figure 2). A first pathway originates from the BLA, and conveys highly integrated polymodal information, including nociceptive, from the thalamus and the cerebral cortex, allowing for example fear conditioning [2, 4, 7]. A cascade of projections, originating in the ventroposterior, posterior, triangular and posterior intralaminar nuclei of the thalamus, the second somatosensory area and the insular cortex, brings nociceptive information to the BLA which in turn transmits it to the CeA [7, 17, 18]. More precisely, the lateral nucleus of the amygdala projects lightly to the CeLC and the CeL and massively to the basolateral nucleus which targets preferentially to the CeM [4, 7, 11]. Additionally, lateral and basolateral projections to the CeA can be relayed in the intercalated cell masses, providing an inhibitory interface in the BLA-CeA pathway [4, 12]. Moreover, the CeA also receives less dense but direct projection from these thalamic and cortical areas [17, 18]. These largely polysynaptic efferents thus allow the CeA to integrate a nociceptive information which already acquired affective and cognitive significance in cortical circuits.

A second pathway conveys to the CeA more direct and raw nociceptive information. While spinal cord only sparsely sends direct projection to the amygdala, spinal nociceptive information is largely transmitted to the CeA via the parabrachial nucleus (PB). This pontine integrative center is a major target for superficial layers of the spinal cord, but also for deeper layers, for the trigeminal complex and for the nucleus of the solitary tract [19, 20]. The nociceptive aspect of these afferents was clearly established by electrophysiology [20]. The PB thus gathers nociceptive information from all cutaneous, deep tissue and visceral territories. Three main ascending pathways are then originating from the PB, toward the medial thalamus, the medial hypothalamus and the central extended amygdala [20]. The parabrachio-amygdaloid pathway is highly organized, with a specific topography connecting various PB subdivisions to the ones of the CeA and the BSTL [19, 20]. Moreover, unitary analysis of parabrachio-amygdaloid axonal branching showed that the different components of the central extended amygdala are innervated by axonal collaterals from a same PB neuronal population that never send projection to the medial thalamus or the medial hypothalamus [21].

In vivo electrophysiological studies allowed characterizing the response of CeA neurons to nociceptive stimulations [6, 19, 20, 22, 23]. In anesthetized rats, cutaneous (mechanical or thermal), deep tissue (articular and muscular) and visceral nociceptive stimulations provoke changes in the activity of a large majority of CeA neurons (Table 1). Most of them, particularly in the CeLC, are activated by nociceptive stimuli, either exclusively or preferentially, while fewer neuronal inhibition can be observed in the CeL. Few CeM neurons are responding to nociceptive stimuli. This has led to define the CeLC as the “nociceptive amygdala” [6] even though contributions from the CeL to nociceptive process cannot be ruled out. The CeA neurons can encode the stimulus intensity, as shown by sigmoid stimulus–response curves. Receptor fields are particularly large, often bilateral and can cover the whole body [20, 22, 23]. These neurons often respond to both cutaneous and deep tissue stimulation, whether mechanical or thermal. Together, these data suggest that even though CeA neurons can detect nociceptive information, the stimulus–response shape of this response as well as the wide receptor fields do not allow the CeA to play a major role in the sensori-discriminative aspect of pain. This brain region rather contributes to pain controls and to the emotional and affective components of pain [5, 6, 19, 20].

Table 1 Reported changes in the rodent CeA in different pain situations

The recruitment of the CeA in visceral and inflammatory pain has also been evidenced using the expression of immediate early genes such as cfos (Table 1). For example, intraperitoneal or esophageal acetic acid injection [24, 25], colorectal distension [42] or experimental cystitis [28] induce cFos in the CeA and the BSTL. Interestingly, this cellular recruitment in the CeA is not observed after intradermal formalin injection in the hindpaw. In this classical inflammatory pain model, cFos recruitment rather appears in the basolateral nucleus of the amygdala [24]. However, extracellular signal-regulated kinase (ERK) phosphorylation is observed in the CeA after intraplantar formalin [30] or in acid-induced muscle pain [32]. Last, in visceral pain models but also in more persistent pain models, such as knee intraarticular injection of kaolin-carrageenan, or neuropathic models based on sciatic nerve ligation, major changes can be observed in the CeA (Table 1), such as an increase in spontaneous and evoked activity [33, 34, 37], an enhancement of synaptic transmission at the PB-CeA and the BLA-CeA synapses [35, 38], an increase in the phosphorylation of NR1 glutamatergic subunit [36], an increased expression of CRF, of group I metabotropic glutamatergic receptors (mGluRs) and of corticosterone receptor [26, 29, 35, 39, 40] and an increased cell proliferation [41].

Human brain imaging confirms a role of the amygdala in pain processes [1]. However, the spatial resolution only allows a crude discrimination of the various nuclei of the amygdaloid complex. Globally, while older studies failed to detect pain-related changes in the amygdala, more recent studies revealed unilateral and bilateral activation or inhibition. These changes were observed after nociceptive thermal laser stimulation [43, 44] or colorectal distention [45]. The amygdala response is usually correlated to the intensity of the nociceptive stimulus, however the importance of attention and emotional factors has also been evidenced. Indeed, repeated thermal nociceptive stimulations of increased intensity lead to an activation of the amygdala that is correlated to the pain perception [43]. However, when sub-threshold stimulus is interpreted as painful by the subject, this situation may activate the amygdala similar to a nociceptive painful stimulus reflecting a state of anxiety or the anticipation of a potentially aversive event. In another study [44], the subjects were informed that they were about to be expose to a cold painful stimulus lasting 1 minute, or 2 minutes. Functional imaging during the first minute of stimulation revealed that the anticipation of longer stimulus duration led to the deactivation of the amygdala. This suggests that the impact of nociceptive information on the amygdala depends on the context, and not simply of the intrinsic properties of the stimulus.

The CeA influences the nociceptive centers

The CeA receives and integrates nociceptive information, but it also influences the main pain centers. The CeA massively projects to other components of the central extended amygdala, to the lateral hypothalamus and to the brainstem [8]. Anatomical data indicate that the intrinsic and the extrinsic projections to the central extended amygdala arise from distinct neuronal populations [11]. Indeed, studies of unitary axonal reconstruction [46] show that medium size spiny neurons of the CeLC and the CeL mostly project to the BSTL and the dorsal substantia innominata, while axons of CeM projection neurons innervate the various brainstem structure through collateral branches. Since the nociceptive inputs from the PB preferentially target the CeLC and the CeL, such organization suggest that nociceptive information are integrated through an interneuron network intrinsic to the CeA and to the central extended amygdala before being delivered to output neurons influencing effector centers of the brainstem. Some of these centers are part of the nociceptive descending controls. Partially opioidergic CeA outputs are for example innervating the PAG that is a key element of descending controls of nociception through its influence on the ventromedial reticular formation [47]. The PB is also part of descending controls and it receives dense inputs from the CeA [8]. Beside these projections toward integrative centers, the CeA also directly projects to the ventromedial reticular formation [48], the dorsal reticular nucleus [49] and monoaminergic centers such as the substantia nigra and the rostral ventral tegmental area (dopamine), the locus coeruleus (noradrenaline) and the raphe nuclei (serotonin) [48, 5052]. These structures are known to influence the nociceptive message. Moreover, monoaminergic centers projecting to the forebrain modulate striatal and cortical activities, potentially influencing affective, emotional and motivational aspects of pain. Finally, within the central extended amygdala, the dorsal substantia innominata that receives strong inputs from the CeA – and particularly from the CeLC – includes cholinergic neurons that innervate the prefrontal and insular cortices [8, 20].

Thus, the CeA has connections allowing the modulation of both the sensory and the affective, emotional and cognitive aspects of pain.

Amygdala, acute pain and analgesia

The potential role of the amygdala in the modulation of pain has been suggested for a long time. It has been clearly shown that the anti- and pro-nociceptive effects are dependent on (1) the type of pain (acute, inflammatory or chronic); (2) the measured parameters (threshold or latency of reflex withdrawal, vocalizations, emotional component); and (3) the emotional state of the subjects (stress, anxiety, fear and expectation).

Nociceptive tests, relying on the latency of appearance of avoidance behavior or on the stimulus threshold, are used to determine the analgesia or hyperalgesia [53]. These tests include for example the tail-flick (tail withdrawal after a thermal stimulus), flinch test (paw or tail withdrawal after electrical stimulation), hot-plate (paw licking or jump after thermal stimulation) or Randall-Selitto (paw withdrawal or vocalization after mechanical stimulation). In these tests, the bilateral lesion or chemical inactivation of the amygdala causes neither anti-nociceptive nor pro-nociceptive effects in naïve animals [5456]. However, injections of neuropeptides such as oxytocin, vasopressin, neurotensin, galanin, CRF or CGRP [5762], or of noradrenergic or cholinergic agonists [6366] into the CeA induce antinociceptive effects (Table 2). In addition, the amygdala is involved in the analgesic action of systemically administered morphine and cannabinoids since the lesion or the inactivation of the CeA strongly reduces the morphine- or cannabinoid-induced analgesia as measured by the tail-flick test [56, 67]. A recent study also showed that deleting the gene of brain-derived neurotrophic factor (BDNF) in parabrachio-amygdaloid neurons decreased the analgesic properties of morphine in mechanical and thermal nociceptive modalities [68]. Finally, the antinociceptive properties of intra-CeA morphine or intra-CeA β-endorphin are dependent upon opioidergic transmission in the amygdala-PAG pathway [47].

Table 2 Effects of CeA manipulation on pain-related outcomes in different pain models

These data therefore show that even if the CeA is not directly involved in the modulation of basal nociceptive thresholds in normal situations, it strongly influences analgesia processes (Table 2).

Stressful situations (restraint or noise) and fear, especially the experimental model of fear conditioning, cause analgesia as measured by the tail flick or the hot-plate tests. Several studies demonstrated that bilateral lesion or inactivation of the CeA diminishes or abolishes this analgesic effect [55, 69, 70, 80, 92] (Table 2). In parallel, clinical studies showed that stressful stimuli, either painful (electric shocks) or painless (unexpected noises), and innocuous stimuli, previously associated with electric shocks (fear conditioned), cause hypoalgesia in a finger withdrawal test following thermal stimulation [93]. In contrast, anxiety caused by electric shock induces hyperalgesia [93]. Although the direct role of the amygdala in these effects has not yet been shown in humans, the results in rodents are consistent with clearly defined functions of the amygdala in emotional responses to different aversive situations [2].

The amygdala thus can participate in adaptive processes leading to alleviation of pain sensation but its role is not limited to antinociception. While CeA lesion can block the antinociception induced in the tail-flick test by electrical shock pre-exposure, the affective hyperalgesia, measured by the latency to vocalize after the shock is also reduced by the same CeA lesion [92]. Interestingly, similar results are observed following lesion of the BSTL [92]. Pharmacological manipulation of the CeA can also increase visceromotor and/or somatomotor reflexes in naïve animals, as shown by intra-CeA injection of agonists to group I mGluRs [76, 77]. Similarly, CGRP and CRF administration in the CeA can induce mechanical hypersensitivity [82, 84], which is in contradiction with older studies reporting antinociceptive effects of these peptides [58, 59] (see below). Finally, corticosterone implants in the CeA sensitize the visceromotor reflexes to colorectal and urinary bladder distension [87, 88] and increases mechanical sensitivity [89]. The amygdala is thus likely to contribute to both analgesia and hyperalgesia.

Amygdala, sustained pain, hyperalgesia and affective component

Persistent pain, such as inflammatory or neuropathic pain, has a different profile than acute pain. While animal studies often don’t address all the symptoms described in humans, the models that are used can display spontaneous nociceptive behaviors, hyperalgesia and/or allodynia, aversion as well as the emotional consequences of pain such as anxiety and depression [94].

The role of the CeA in sustained pain has been examined after intraplantar injection of formalin (somatic inflammatory pain), intraperitoneal injection of acetic acid (visceral inflammatory pain), intraarticular injection of kaolin and carrageenan (somatic inflammatory pain), and ligation/compression of the sciatic nerve (neuropathic pain) (Table 2). Generally, the manipulation of CeA activity, either by activation or by inactivation, did not modify the spontaneous nociceptive behaviors [30, 71, 72]. However, persistent pain leads to increased neuronal activity and synaptic transmission in the CeA (Table 1) which may be involved in the induction and/or maintenance of hypersensitivity observed in these models [5, 6, 33, 38]. Thus, inhibiting the extracellular signal-regulated kinase (ERK) activation in the CeA decreases mechanical, but not thermal, hyperalgesia in formalin and arthritis models, while the direct activation of ERK in the CeA is sufficient to produce mechanical hyperalgesia in naïve animals [30, 91]. In addition, intra-CeA injections of mGluRs antagonist (groups I and III) or of CGRP1 or CRF1 antagonists alleviate the increased withdrawal reflexes as well as audible and ultrasonic vocalizations in monoarthritic animals [78, 79, 83, 85, 86]. In neuropathic rats, activation of GABA-A receptors in the CeA also diminishes mechanical hypersensitivity [74]. These data suggest that changes in the activity and neurochemistry of the CeA contribute to the exacerbation of nociceptive responses in persistent pain.

The affective and emotional dimensions of pain can also be under the influence of the amygdala. The effects of pharmacological manipulations of the CeA on vocalizations reported above (see also Table 1) are pertinent in this context. Indeed, while audible vocalizations induced by a nociceptive stimulus are believed to reflect nocifensive responses, ultrasonic vocalizations would reflect an affective pain response [78, 95]. A widely used test to assess the affective/emotional component of pain, is the pain-induced conditioned place aversion. This behavior is suppressed in the intraplantar formalin and intraperitoneal acetic acid models by bilateral lesions of the CeA [72, 73] or by injection of β-adrenoceptor antagonist [81]. Similarly, intra-CeA administration of a GABA-A receptor agonist, a NMDA antagonist or a group I mGluR antagonist reduces the place avoidance behavior in neuropathic rats [74, 75].

Finally, the CeA may be a part of the mechanism linking sustained pain to anxiety and depression-like states. It has been shown that the models of monoarthritic pain or of sciatic nerve ligation induce anxiety that is correlated with the activation of the CRF system in the CeA. The expression of this neuropeptide, which has a well established role in anxiety, is increased in the CeA of neuropathic animals [39, 40], while the intra-CeA injection of CRF1 antagonist reduces anxious behaviors produced by knee monoarthritis [85, 86]. In addition, the pronociceptive effects of corticosterone implants in the CeA are accompanied by anxiety-like behaviors [90].

In humans, brain imaging studies [1] demonstrated changes in the activity of the amygdala of patients with irritable bowel syndrome [45], arthritis [96] or mononeuropathy [97], suggesting that the amygdala may be involved in the emotional aspects related to these pathologies. Interestingly, a brain imaging study showed the participation of an amygdala - anterior cingulate cortex circuit in the higher subjective perception of pain in healthy subjects experiencing sadness [98]. Conversely, viewing pictures of a romantic partner reduced self-reported pain, in association with activation of the amygdala [99]. These data reinforce the role of amygdala in emotional impact on pain.

In summary, the amygdala, particularly the CeA, may have a mainly antinociceptive influence in acute/phasic pain conditions associated with situations of stress or fear, and a mainly pronociceptive influence in persistent/tonic pain conditions which concerns the sensory as well as the emotional and affective dimensions.

Potential mechanisms with several actors

Some information is available on the cellular and molecular mechanisms by which the amygdala can exert its bidirectional effects on pain parameters. Projections from the PB to the CeA give direct information about the perceived nociceptive stimulation through glutamatergic and peptidergic synapses [100102], while the glutamatergic projections from the BLA to the CeA can bring polymodal information with an affective valence [5]. This latter input can be turned into an inhibitory control through the intercalated cell masses, functionally placed between the BLA and the CeA [4, 12]. The influence of the CeA on its targets will depend on the confrontation of these inputs with other afferent information. The internal network of the CeA and of the central extended amygdala is based on GABAergic inhibitory neurons [103], and the output of this system can either be inhibitory or disinhibitory on target structures [4, 104, 105]. Changes in the level of pain perception seem to involve the projection of the CeA to the centers of descending pain control, especially the PAG and the ventromedial reticular formation [47], while the influence of the amygdala on the emotional and affective dimensions of pain arises from a larger network that involves indirect connections with cortical regions such as the insular and the cingulate cortex [5, 20].

It is now clearly established that persistent pain causes long-lasting changes in the activity of the CeA that could account for its pronociceptive influence on sensory and affective components of pain [5, 6]. In arthritic, visceral and neuropathic pain models, subpopulations of nociceptive neurons of the CeA exhibit increased membrane excitability, leading to higher spontaneous activity, as well as potentiation of synaptic transmission [27, 33, 35, 37, 38]. This synaptic plasticity, observed at both the PB-CeA and the BLA-CeA synapses can potentiate nociceptive transmission. Some specificity can be observed concerning pain-related plasticity in the CeA. Indeed, in arthritis model, the population of multireceptive neurons, responding to innocuous stimuli but preferentially to nociceptive stimuli, presents increased basal activity and increased responses to mechanical but not thermal nociceptive stimuli. A second neuronal population, normally insensitive to somatosensory stimulation, develops responses to mechanical, but not thermal nociceptive stimuli. In contrast, arthritis modifies neither the basal activity nor the responses of the nociceptive-specific neurons [33]. In addition, while the PB and the BLA can synapse onto the same neurons, but on different dendritic compartments [106], specific plastic changes can occurs with different pain models. While arthritic and neuropathic pain models potentiates both the PB-CeA and the BLA-CeA synapses [33, 38], a model of visceral pain only potentiates the PB-CeA synapses [27].

An intriguing observation is that the right CeA appears largely more implicated in persistent pain than the left CeA. Indeed, the activation of ERK after intraplantar formalin is restricted to the right CeA, and the blockade of ERK activation in the right CeA decreased mechanical hyperalgesia at both hindpaws, irrespective to the side of formalin injection [30, 31]. In the arthritis model, the enhanced background activity and evoked responses are observed only in the right CeA, as well as the decreased activity following injection of a PKA inhibitor [34]. Finally, in neuropathic rats, spontaneous activity and evoked responses increased in the left CeA 2 and 6 days after sciatic nerve ligation, but declined afterward, whereas these electrophysiological parameters increased in the right CeA at 14 days post-ligation [37]. The different implication of left and right CeA in pain processes could partially account for the conflicting reports indicating antinociceptive [58, 59] and pronociceptive [82, 84] effects of CRF and CGRP injected into the CeA of naive rats. The anatomical and functional basis of this lateralization remains unclear, but it suggests a strong relation between right amygdala and affective/emotional component of persistent pain.

A number of neurochemical and molecular mechanisms involved in the sensitization of the CeA have been elucidated. They especially implicate the modification of pre-and post-synaptic glutamatergic transmission, with an impact on GABAergic transmission, along with neuropeptidergic modulation [5, 6] (Figure 3). Following induction of experimental arthritis, the pre-synaptic expression of mGluR1 (group I) is increased, which in turn potentiates the synaptic transmission [35, 107]. This effect seems to involve a decreased inhibition, resulting in disinhibition of CeLC neurons and of glutamatergic terminals [108]. On the other hand, the activation of group II and III pre-synaptic mGluRs diminishes synaptic transmission [109111]. At post-synaptic level, activation of group I mGluR (mGluR5) in the CeLC increased the excitatory response to nociceptive input in normal condition but not in arthritic condition [108], possibly involving PKA and ERK activation through reactive oxygen species [112]. In the same inflammatory pain model, NMDA ionotropic receptors are implicated in post-synaptic a sensitization, via a PKA-dependant mechanism [36, 113], while a NMDA-independent synaptic plasticity is induced in a neuropathic pain model [38]. Last, neuropeptide receptors (CRF1, CGRP1) contribute to this plasticity by favoring NMDA receptor phosphorylation through the recruitment of PKA and ERK kinases [8284]. Together, these mechanisms lead to both increased nociceptive transmission and neuron sensitization in the CeA. The implication of CRF is here important as it also plays a role in stress response and anxiety. Moreover, while CGRP and CRF in CeA have a pronociceptive influence in arthritis model, the same neuropeptides can be acutely antinociceptive when directly injected in the CeA of control animals [58, 59]. This dual action, which likely implies qualitative and quantitative receptor changes, may also exist for other neuropeptides present in CeA, such as oxytocin or neurotensin. Opioid system in the CeA may be important in these phenomenons, but its contributions still remain elusive. The three types of opioid receptors (mu, delta, kappa) are expressed in the CeA, both pre- and post-synaptically, as well as their endogenous ligands [15, 16, 114116]. The opioid system is a key actor in the CeA, due to the enkephalin contribution to intrinsic communications within the central extended amygdala and due to the contribution of the other opioid peptides to extrinsic CeA outputs [11]. The influence of the opioid system in the amygdala on pain is supported by various data, including the antinociceptive action of morphine in the CeA, the synergy between the CeA and the PAG co-delivery of opioid ligands [47] and the changes in CeA opioid receptor binding in neuropathic mice [117]. However, the circuit architecture underlying these actions, and the individual role of each peptide and receptor, is not yet clearly defined. For example, the mu-opioid receptors are pre-synaptically present in the CeA, where they can inhibit glutamate and GABA release from afferents [115, 118], but they also act post-synaptically to inhibit CeA interneurons and projection neurons [116] (Figure 3). Thus, these receptors can either inhibit or activate each component of the CeA. It is also not yet known how the opioid system interacts with other neurotransmission systems within the CeA, but several studies suggest that the CeA opioid system act downstream to agents that promote antinociception such as galanin or BDNF [60, 68]. Finally, adrenoceptors modulate pain processes in the CeA [64, 80, 81], especially through α2 adrenoceptors located on PB-CeA pre-synaptic elements which strongly regulate glutamate release [100].

Figure 3
figure 3

Molecular actors in nociceptive processing in the CeA during arthritic pain. The CeA received nociceptive inputs from glutamatergic (Glu) neurons in the basolateral amygdala (BLA) and in the parabrachial nucleus (PB). GABAergic innervation originates from intrinsic source (CeA and BSTL) and from the intercalated cell masses (ITC). Additional modulation is provided by monoaminergic (MA) and cholinergic (ACh) afferents and by neuropeptides (Np), especially from CeA, BSTL and PB. These neuromediators act on excitatory (green) and inhibitory (red) receptors at pre and post-synaptic levels. In the knee monoarthritis model [5], several mechanisms contribute to a enhanced neurotransmission. At pre-synaptic level, mGluR1 receptors inhibit GABA release, while mGluR from groups II and III decrease glutamate release. At post-synaptic level, mGluR5 receptors are involved in normal transmission in naive animals and NMDA and AMPA ionotropic receptors have also an enhanced activity increasing neuronal sensitivity. CGRP, from PB, and CRF, possibly from the central extended amygdala itself, activate their post-synaptic receptors, CGRP1 and CRF1, respectively. These receptors can activate the protein kinase A (PKA) and thus increase NMDA phosphorylation. The extracellular regulated-signal kinase ERK also contributes to the sensitization of CeA neurons, possibly through a cascade initiated by mGluR5 receptors. CRF can also decrease the activity of CeA neurons, via pre-synaptic CRF2 receptors increasing GABA release. This leads to the inhibition of CeA neurons by activation of post-synaptic GABA A and GABA B receptors and by inhibition of glutamate release by pre-synaptic GABA B receptors. Other neurotransmission system involving noradrenergic (NA, especially through pre-synaptic α2-receptors), dopaminergic (DA), serotonergic (5-HT) and cholinergic (ACh) receptors can modulate CeA activity, as well as other neuropeptides, among them opioids acting on pre- and post-synaptic mu receptors (MOR), and BDNF acting on TrkB receptors. Overall, these mechanisms lead to the modulation of the intra-CeA circuitry, based on GABAergic interactions (in blue) and of the CeA output.

Beyond the central amygdala

While we focused this review on the CeA, it is necessary to remind the contribution of BSTL and BLA to pain process.

The CeA belongs to the central extended amygdala; it is largely interconnected with the BSTL and shares similar afferents, including polymodal and nociceptive inputs from the BLA and the PB [8, 21]. While there is no report of an influence of the BSTL on nociceptive sensitivity, a few studies addressed its role in pain-induced conditioned place aversion. Bilateral lesion of the BSTL [119], as well as intra-BSTL injection of a β-adrenoceptor antagonist [120, 121], α2-adrenoceptor agonist [122] or CRF1 antagonist [123] decrease place aversion in the intraplantar formalin and/or intraperitoneal acetic acid models. Moreover, noradrenaline and CRF release in the BSTL is enhanced in these pain models [120122] and CRF mRNA is upregulated in the BSTL of neuropathic rats [40]. These data suggest that the BSTL can be involved in affective/emotional component of pain, maybe in a complementary manner with the CeA, especially in view of BSTL implication in anxiety [3].

The BLA, beyond its role as a mere input provider to the CeA and BSTL, also appears as an important actor in pain processes. Some observations suggest that the BLA and the CeA can have a parallel role in nocifensive responses. Stress- and fear-induced analgesia are reduced by intra-BLA infusion of cannabinoid CB1 receptors antagonists, of muscimol or of diazepam [124127]; mu opioid agonist, including morphine, injected in the BLA decreases vocalizations to tail shocks [128] and thermal nociceptive sensitivity [129, 130]. Sustained pain also impacts the BLA as intraplantar formalin induces c-fos mRNA [24], arthritis increases the expression of the pro-nociceptive cytokine tumor necrosis factor α (TNF-α) [131] and neuropathy increases cell proliferation in the BLA [41]. A functional plasticity of BLA neurons is also observed in arthritis model, characterized by an increased in spontaneous and evoked activity and enhanced synaptic transmission. This pain-induced plasticity can be reversed by CRF1 antagonism [132].

The affective component of pain can also be modulated by the BLA. The formalin-induced conditioned place aversion is reduced by lesion of the BLA [72], as it is the case for the CeA and the BSTL, but also by BLA injection of NMDA antagonist or of morphine [133]. In addition, neutralizing BLA TNF-α with Infliximab antibodies reduced anxiety-like behaviors associated to arthritis [131]. Finally, CRF1 antagonism in BLA of arthritic rats decreased mechanical hypersensitivity, but also pain-related vocalizations and anxiety [132].

These influences of BLA on sensory and affective/emotional aspects of pain can, at least in part, involve its outputs to the CeA and/or BSTL. However, the BLA also appears to be implicated in pain-related cognitive impairment. Indeed, in arthritic rats, blockade of pain-induced plasticity in the BLA with CRF1 antagonism reversed the deficit in decision making evaluated in a gambling task. This deficit has been shown to be dependent upon a deactivation of the medial prefrontal cortex driven by the hyperactivity in the BLA. Importantly, this mechanism appears independent of the CeA [132]. Thus, the connections of the BLA with the cerebral cortex, especially prefrontal, cingulate and insular cortices, could allow the amygdala to influence both the affective/emotional and the cognitive aspects of pain. In addition, the dense BLA input to the ventral striatum [9, 10] could also participates in pain-induced changes in motivation and goal-directed behaviors.

Conclusions

The data from the literature show that the amygdala, and in particular the CeA, contributes to pain processes. Its anatomical and functional relations with pain ascending systems, with pain facilitating or inhibitory descending systems, and with affective and cognitive centers are placing the CeA in a critical position within the pain matrix. Acute pain reactions only require CeA if they occur in specific emotional or adaptive contexts. On the other side, persistent and chronic pains alter CeA activity, which influences the pain experience and the related emotional, affective and motivational states. Reciprocally, these states can modify the amygdala processing of information and its impact on pain.

The amygdala has been mostly considered for its roles in emotion, especially in fear [2, 4]. While most studies focused on the BLA, recent researches on the fear circuit disclosed a part of the CeA microcircuitry involved in fear conditioning [104, 105]. It is very likely that a similar, if not the same, circuit underlies CeA functions in pain. Indeed, a recent study showed for the first time that the “nociceptive” amygdala (CeLC) undergoes synaptic plasticity at the PB-CeA and the BLA-CeA synapses during fear conditioning [134]. This result emphasizes the need to consider the strong relation between pain and emotion. The contribution to the pain matrix of the CeA, of other components of the amygdala and of the central extended amygdala, should foster researches in the context of chronic pain and the associated anxio-depressive disorders.

Abbreviations

BLA:

Basolateral amygdala

CeA:

Central amygdaloid nucleus

CeL:

Lateral part of the central amygdaloid nucleus

CeLC:

Capsular part of the central amygdaloid nucleus

CeM:

Medial part of the central amygdaloid nucleus

CGRP:

Calcitonin gene-related peptide

CRF:

Corticotropin-releasing factor

ERK:

Extracellular signal-regulated kinase

mGluR:

Metabotropic glutamatergic receptor

PB:

Parabrachial nucleus

PKA:

Protein kinase A

TNF-α:

Tumor necrosis factor α.

References

  1. Simons LE, Moulton EA, Linnman C, Carpino E, Becerra L, Borsook D: The human amygdala and pain: evidence from neuroimaging. Hum Brain Mapp 2012. in press 10.1002/hbm.22199

    Google Scholar 

  2. LeDoux JE: Emotion circuits in the brain. Annu Rev Neurosci 2000, 23: 155–184. 10.1146/annurev.neuro.23.1.155

    Article  CAS  PubMed  Google Scholar 

  3. Davis M, Walker DL, Miles L, Grillon C: Phasic vs sustained fear in rats and humans: role of the extended amygdala in fear vs anxiety. Neuropsychopharmacology 2010, 35: 105–135. 10.1038/npp.2009.109

    Article  PubMed Central  PubMed  Google Scholar 

  4. Pare D, Duvarci S: Amygdala microcircuits mediating fear expression and extinction. Curr Opin Neurobiol 2012, 22: 717–723. 10.1016/j.conb.2012.02.014

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  5. Neugebauer V, Galhardo V, Maione S, Mackey SC: Forebrain pain mechanisms. Brain Res Rev 2009, 60: 226–242. 10.1016/j.brainresrev.2008.12.014

    Article  PubMed Central  PubMed  Google Scholar 

  6. Neugebauer V, Li W, Bird GC, Han JS: The amygdala and persistent pain. Neuroscientist 2004, 10: 221–234. 10.1177/1073858403261077

    Article  PubMed  Google Scholar 

  7. Sah P, Faber ES, Lopez De Armentia M, Power J: The amygdaloid complex: anatomy and physiology. Physiol Rev 2003, 83: 803–834.

    Article  CAS  PubMed  Google Scholar 

  8. Alheid GF, de Olmos JS, Beltramino CA: Amygdala and extended amygdala. In The Rat Nervous System. 2nd edition. Edited by: Paxinos G. San Diego, CA: Academic; 1995:495–578.

    Google Scholar 

  9. Buot A, Yelnik J: Functional anatomy of the basal ganglia: limbic aspects. Rev Neurol (Paris) 2012, 168: 569–575. 10.1016/j.neurol.2012.06.015

    Article  CAS  Google Scholar 

  10. Fudge JL, Breitbart MA, McClain C: Amygdaloid inputs define a caudal component of the ventral striatum in primates. J Comp Neurol 2004, 476: 330–347. 10.1002/cne.20228

    Article  PubMed Central  PubMed  Google Scholar 

  11. Cassell MD, Freedman LJ, Shi C: The intrinsic organization of the central extended amygdala. Ann N Y Acad Sci 1999, 877: 217–241. 10.1111/j.1749-6632.1999.tb09270.x

    Article  CAS  PubMed  Google Scholar 

  12. Palomares-Castillo E, Hernandez-Perez OR, Perez-Carrera D, Crespo-Ramirez M, Fuxe K, Perez de la Mora M: The intercalated paracapsular islands as a module for integration of signals regulating anxiety in the amygdala. Brain Res 2012, 1476: 211–234.

    Article  CAS  PubMed  Google Scholar 

  13. Heimer L, Van Hoesen GW, Trimble M, Zahm DS: Anatomy of neuropsychiatry: the new anatomy of the basal forebrain and its implications for neuropsychiatric illness. Amsterdam: Boston Academic Press/Elsevier; 2008.

    Google Scholar 

  14. Veinante P, Stoeckel ME, Freund-Mercier MJ: GABA- and peptide-immunoreactivities co-localize in the rat central extended amygdala. NeuroReport 1997, 8: 2985–2989. 10.1097/00001756-199709080-00035

    Article  CAS  PubMed  Google Scholar 

  15. Poulin JF, Castonguay-Lebel Z, Laforest S, Drolet G: Enkephalin co-expression with classic neurotransmitters in the amygdaloid complex of the rat. J Comp Neurol 2008, 506: 943–959. 10.1002/cne.21587

    Article  CAS  PubMed  Google Scholar 

  16. Marchant NJ, Densmore VS, Osborne PB: Coexpression of prodynorphin and corticotrophin-releasing hormone in the rat central amygdala: evidence of two distinct endogenous opioid systems in the lateral division. J Comp Neurol 2007, 504: 702–715. 10.1002/cne.21464

    Article  CAS  PubMed  Google Scholar 

  17. McDonald AJ, Shammah-Lagnado SJ, Shi C, Davis M: Cortical afferents to the extended amygdala. Ann N Y Acad Sci 1999, 877: 309–338. 10.1111/j.1749-6632.1999.tb09275.x

    Article  CAS  PubMed  Google Scholar 

  18. Shi C, Davis M: Pain pathways involved in fear conditioning measured with fear-potentiated startle: lesion studies. J Neurosci 1999, 19: 420–430.

    CAS  PubMed  Google Scholar 

  19. Bernard JF, Bester H, Besson JM: Involvement of the spino-parabrachio -amygdaloid and -hypothalamic pathways in the autonomic and affective emotional aspects of pain. Prog Brain Res 1996, 107: 243–255.

    Article  CAS  PubMed  Google Scholar 

  20. Gauriau C, Bernard JF: Pain pathways and parabrachial circuits in the rat. Exp Physiol 2002, 87: 251–258. 10.1113/eph8702357

    Article  PubMed  Google Scholar 

  21. Sarhan M, Freund-Mercier MJ, Veinante P: Branching patterns of parabrachial neurons projecting to the central extended amygdala: single axonal reconstructions. J Comp Neurol 2005, 491: 418–442. 10.1002/cne.20697

    Article  PubMed  Google Scholar 

  22. Bernard JF, Huang GF, Besson JM: Nucleus centralis of the amygdala and the globus pallidus ventralis: electrophysiological evidence for an involvement in pain processes. J Neurophysiol 1992, 68: 551–569.

    CAS  PubMed  Google Scholar 

  23. Neugebauer V, Li W: Processing of nociceptive mechanical and thermal information in central amygdala neurons with knee-joint input. J Neurophysiol 2002, 87: 103–112.

    PubMed  Google Scholar 

  24. Nakagawa T, Katsuya A, Tanimoto S, Yamamoto J, Yamauchi Y, Minami M, Satoh M: Differential patterns of c-fos mRNA expression in the amygdaloid nuclei induced by chemical somatic and visceral noxious stimuli in rats. Neurosci Lett 2003, 344: 197–200. 10.1016/S0304-3940(03)00465-8

    Article  CAS  PubMed  Google Scholar 

  25. Suwanprathes P, Ngu M, Ing A, Hunt G, Seow F: c-Fos immunoreactivity in the brain after esophageal acid stimulation. Am J Med 2003,115(Suppl 3A):31S-38S.

    Article  CAS  PubMed  Google Scholar 

  26. Greenwood-Van Meerveld B, Johnson AC, Schulkin J, Myers DA: Long-term expression of corticotropin-releasing factor (CRF) in the paraventricular nucleus of the hypothalamus in response to an acute colonic inflammation. Brain Res 2006, 1071: 91–96. 10.1016/j.brainres.2005.11.071

    Article  CAS  PubMed  Google Scholar 

  27. Han JS, Neugebauer V: Synaptic plasticity in the amygdala in a visceral pain model in rats. Neurosci Lett 2004, 361: 254–257. 10.1016/j.neulet.2003.12.027

    Article  CAS  PubMed  Google Scholar 

  28. Bon K, Lanteri-Minet M, Michiels JF, Menetrey D: Cyclophosphamide cystitis as a model of visceral pain in rats: a c-fos and Krox-24 study at telencephalic levels, with a note on pituitary adenylate cyclase activating polypeptide (PACAP). Exp Brain Res 1998, 122: 165–174. 10.1007/s002210050504

    Article  CAS  PubMed  Google Scholar 

  29. Nishii H, Nomura M, Aono H, Fujimoto N, Matsumoto T: Up-regulation of galanin and corticotropin-releasing hormone mRNAs in the key hypothalamic and amygdaloid nuclei in a mouse model of visceral pain. Regul Pept 2007, 141: 105–112. 10.1016/j.regpep.2006.12.022

    Article  CAS  PubMed  Google Scholar 

  30. Carrasquillo Y, Gereau RW: Activation of the extracellular signal-regulated kinase in the amygdala modulates pain perception. J Neurosci 2007, 27: 1543–1551. 10.1523/JNEUROSCI.3536-06.2007

    Article  CAS  PubMed  Google Scholar 

  31. Carrasquillo Y, Gereau RW: Hemispheric lateralization of a molecular signal for pain modulation in the amygdala. Mol Pain 2008, 4: 24. 10.1186/1744-8069-4-24

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  32. Cheng SJ, Chen CC, Yang HW, Chang YT, Bai SW, Yen CT, Min MY: Role of extracellular signal-regulated kinase in synaptic transmission and plasticity of a nociceptive input on capsular central amygdaloid neurons in normal and acid-induced muscle pain mice. J Neurosci 2011, 31: 2258–2270. 10.1523/JNEUROSCI.5564-10.2011

    Article  CAS  PubMed  Google Scholar 

  33. Neugebauer V, Li W: Differential sensitization of amygdala neurons to afferent inputs in a model of arthritic pain. J Neurophysiol 2003, 89: 716–727.

    Article  PubMed  Google Scholar 

  34. Ji G, Neugebauer V: Hemispheric lateralization of pain processing by amygdala neurons. J Neurophysiol 2009, 102: 2253–2264. 10.1152/jn.00166.2009

    Article  PubMed Central  PubMed  Google Scholar 

  35. Neugebauer V, Li W, Bird GC, Bhave G, Gereau RW: Synaptic plasticity in the amygdala in a model of arthritic pain: differential roles of metabotropic glutamate receptors 1 and 5. J Neurosci 2003, 23: 52–63.

    CAS  PubMed  Google Scholar 

  36. Bird GC, Lash LL, Han JS, Zou X, Willis WD, Neugebauer V: Protein kinase A-dependent enhanced NMDA receptor function in pain-related synaptic plasticity in rat amygdala neurones. J Physiol 2005, 564: 907–921. 10.1113/jphysiol.2005.084780

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  37. Goncalves L, Dickenson AH: Asymmetric time-dependent activation of right central amygdala neurones in rats with peripheral neuropathy and pregabalin modulation. Eur J Neurosci 2012, 36: 3204–3213. 10.1111/j.1460-9568.2012.08235.x

    Article  PubMed  Google Scholar 

  38. Ikeda R, Takahashi Y, Inoue K, Kato F: NMDA receptor-independent synaptic plasticity in the central amygdala in the rat model of neuropathic pain. Pain 2007, 127: 161–172. 10.1016/j.pain.2006.09.003

    Article  CAS  PubMed  Google Scholar 

  39. Ulrich-Lai YM, Xie W, Meij JT, Dolgas CM, Yu L, Herman JP: Limbic and HPA axis function in an animal model of chronic neuropathic pain. Physiol Behav 2006, 88: 67–76. 10.1016/j.physbeh.2006.03.012

    Article  CAS  PubMed  Google Scholar 

  40. Rouwette T, Vanelderen P, de Reus M, Loohuis NO, Giele J, van Egmond J, Scheenen W, Scheffer GJ, Roubos E, Vissers K, Kozicz T: Experimental neuropathy increases limbic forebrain CRF. Eur J Pain 2012, 16: 61–71. 10.1016/j.ejpain.2011.05.016

    Article  CAS  PubMed  Google Scholar 

  41. Goncalves L, Silva R, Pinto-Ribeiro F, Pego JM, Bessa JM, Pertovaara A, Sousa N, Almeida A: Neuropathic pain is associated with depressive behaviour and induces neuroplasticity in the amygdala of the rat. Exp Neurol 2008, 213: 48–56. 10.1016/j.expneurol.2008.04.043

    Article  PubMed  Google Scholar 

  42. Traub RJ, Silva E, Gebhart GF, Solodkin A: Noxious colorectal distention induced-c-Fos protein in limbic brain structures in the rat. Neurosci Lett 1996, 215: 165–168. 10.1016/0304-3940(96)12978-5

    Article  CAS  PubMed  Google Scholar 

  43. Bornhovd K, Quante M, Glauche V, Bromm B, Weiller C, Buchel C: Painful stimuli evoke different stimulus–response functions in the amygdala, prefrontal, insula and somatosensory cortex: a single-trial fMRI study. Brain 2002, 125: 1326–1336. 10.1093/brain/awf137

    Article  CAS  PubMed  Google Scholar 

  44. Petrovic P, Carlsson K, Petersson KM, Hansson P, Ingvar M: Context-dependent deactivation of the amygdala during pain. J Cogn Neurosci 2004, 16: 1289–1301. 10.1162/0898929041920469

    Article  PubMed  Google Scholar 

  45. Bonaz B, Baciu M, Papillon E, Bost R, Gueddah N, Le Bas JF, Fournet J, Segebarth C: Central processing of rectal pain in patients with irritable bowel syndrome: an fMRI study. Am J Gastroenterol 2002, 97: 654–661. 10.1111/j.1572-0241.2002.05545.x

    Article  CAS  PubMed  Google Scholar 

  46. Veinante P, Freund-Mercier MJ: Branching patterns of central amygdaloid nucleus efferents in the Rat: single-axon reconstructions. Ann N Y Acad Sci 2003, 985: 552–553.

    Article  Google Scholar 

  47. Pavlovic ZW, Bodnar RJ: Opioid supraspinal analgesic synergy between the amygdala and periaqueductal gray in rats. Brain Res 1998, 779: 158–169. 10.1016/S0006-8993(97)01115-3

    Article  CAS  PubMed  Google Scholar 

  48. Hermann DM, Luppi PH, Peyron C, Hinckel P, Jouvet M: Afferent projections to the rat nuclei raphe magnus, raphe pallidus and reticularis gigantocellularis pars alpha demonstrated by iontophoretic application of choleratoxin (subunit b). J Chem Neuroanat 1997, 13: 1–21. 10.1016/S0891-0618(97)00019-7

    Article  CAS  PubMed  Google Scholar 

  49. Almeida A, Cobos A, Tavares I, Lima D: Brain afferents to the medullary dorsal reticular nucleus: a retrograde and anterograde tracing study in the rat. Eur J Neurosci 2002, 16: 81–95. 10.1046/j.1460-9568.2002.02058.x

    Article  PubMed  Google Scholar 

  50. Jin X, Cui N, Zhong W, Jin XT, Jiang C: GABA-ergic synaptic inputs of locus coeruleus neurons in wild-type and Mecp2-null mice. Am J Physiol Cell Physiol 2013. in press 10.1152/ajpcell.00399.2012

    Google Scholar 

  51. Zahm DS, Cheng AY, Lee TJ, Ghobadi CW, Schwartz ZM, Geisler S, Parsely KP, Gruber C, Veh RW: Inputs to the midbrain dopaminergic complex in the rat, with emphasis on extended amygdala-recipient sectors. J Comp Neurol 2011, 519: 3159–3188. 10.1002/cne.22670

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  52. Wallace DM, Magnuson DJ, Gray TS: Organization of amygdaloid projections to brainstem dopaminergic, noradrenergic, and adrenergic cell groups in the rat. Brain Res Bull 1992, 28: 447–454. 10.1016/0361-9230(92)90046-Z

    Article  CAS  PubMed  Google Scholar 

  53. Barrot M: Tests and models of nociception and pain in rodents. Neuroscience 2012, 211: 39–50.

    Article  CAS  PubMed  Google Scholar 

  54. Calvino B, Levesque G, Besson JM: Possible involvement of the amygdaloid complex in morphine analgesia as studied by electrolytic lesions in rats. Brain Res 1982, 233: 221–226. 10.1016/0006-8993(82)90946-5

    Article  CAS  PubMed  Google Scholar 

  55. Helmstetter FJ, Bellgowan PS: Lesions of the amygdala block conditional hypoalgesia on the tail flick test. Brain Res 1993, 612: 253–257. 10.1016/0006-8993(93)91669-J

    Article  CAS  PubMed  Google Scholar 

  56. Manning BH, Mayer DJ: The central nucleus of the amygdala contributes to the production of morphine antinociception in the rat tail-flick test. J Neurosci 1995, 15: 8199–8213.

    CAS  PubMed  Google Scholar 

  57. Han Y, Yu LC: Involvement of oxytocin and its receptor in nociceptive modulation in the central nucleus of amygdala of rats. Neurosci Lett 2009, 454: 101–104. 10.1016/j.neulet.2009.02.062

    Article  CAS  PubMed  Google Scholar 

  58. Cui XY, Lundeberg T, Yu LC: Role of corticotropin-releasing factor and its receptor in nociceptive modulation in the central nucleus of amygdala in rats. Brain Res 2004, 995: 23–28. 10.1016/j.brainres.2003.09.050

    Article  CAS  PubMed  Google Scholar 

  59. Xu W, Lundeberg T, Wang YT, Li Y, Yu LC: Antinociceptive effect of calcitonin gene-related peptide in the central nucleus of amygdala: activating opioid receptors through amygdala-periaqueductal gray pathway. Neuroscience 2003, 118: 1015–1022. 10.1016/S0306-4522(03)00069-1

    Article  CAS  PubMed  Google Scholar 

  60. Jin WY, Liu Z, Liu D, Yu LC: Antinociceptive effects of galanin in the central nucleus of amygdala of rats, an involvement of opioid receptors. Brain Res 2010, 1320: 16–21.

    Article  CAS  PubMed  Google Scholar 

  61. Ahn DK, Kim KH, Ju JS, Kwon S, Park JS: Microinjection of arginine vasopressin into the central nucleus of amygdala suppressed nociceptive jaw opening reflex in freely moving rats. Brain Res Bull 2001, 55: 117–121. 10.1016/S0361-9230(01)00493-2

    Article  CAS  PubMed  Google Scholar 

  62. Kalivas PW, Gau BA, Nemeroff CB, Prange AJ Jr: Antinociception after microinjection of neurotensin into the central amygdaloid nucleus of the rat. Brain Res 1982, 243: 279–286. 10.1016/0006-8993(82)90251-7

    Article  CAS  PubMed  Google Scholar 

  63. Ahn DK, Kim YS, Park JS: Central-amygdaloid carbachol suppressed nociceptive jaw opening reflex in freely moving rats. Prog Neuropsychopharmacol Biol Psychiatry 1999, 23: 685–695. 10.1016/S0278-5846(99)00027-5

    Article  CAS  PubMed  Google Scholar 

  64. Ortiz JP, Heinricher MM, Selden NR: Noradrenergic agonist administration into the central nucleus of the amygdala increases the tail-flick latency in lightly anesthetized rats. Neuroscience 2007, 148: 737–743. 10.1016/j.neuroscience.2007.07.003

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  65. Oliveira MA, Prado WA: Antinociception and behavioral manifestations induced by intracerebroventricular or intra-amygdaloid administration of cholinergic agonists in the rat. Pain 1994, 57: 383–391. 10.1016/0304-3959(94)90014-0

    Article  CAS  PubMed  Google Scholar 

  66. Leite-Panissi CR, Brentegani MR, Menescal-de-Oliveira L: Cholinergic-opioidergic interaction in the central amygdala induces antinociception in the guinea pig. Braz J Med Biol Res 2004, 37: 1571–1579. 10.1590/S0100-879X2004001000018

    Article  CAS  PubMed  Google Scholar 

  67. Manning BH, Martin WJ, Meng ID: The rodent amygdala contributes to the production of cannabinoid-induced antinociception. Neuroscience 2003, 120: 1157–1170. 10.1016/S0306-4522(03)00356-7

    Article  CAS  PubMed  Google Scholar 

  68. Sarhan M, Pawlowski SA, Barthas F, Yalcin I, Kaufling J, Dardente H, Zachariou V, Dileone RJ, Barrot M, Veinante P: BDNF parabrachio-amygdaloid pathway in morphine-induced analgesia. Int J Neuropsychopharmacol 2013. in press 10.1017/S146114571200168X

    Google Scholar 

  69. Bellgowan PS, Helmstetter FJ: Neural systems for the expression of hypoalgesia during nonassociative fear. Behav Neurosci 1996, 110: 727–736.

    Article  CAS  PubMed  Google Scholar 

  70. Helmstetter FJ: The amygdala is essential for the expression of conditional hypoalgesia. Behav Neurosci 1992, 106: 518–528.

    Article  CAS  PubMed  Google Scholar 

  71. Manning BH, Mayer DJ: The central nucleus of the amygdala contributes to the production of morphine antinociception in the formalin test. Pain 1995, 63: 141–152. 10.1016/0304-3959(95)00027-P

    Article  CAS  PubMed  Google Scholar 

  72. Tanimoto S, Nakagawa T, Yamauchi Y, Minami M, Satoh M: Differential contributions of the basolateral and central nuclei of the amygdala in the negative affective component of chemical somatic and visceral pains in rats. Eur J Neurosci 2003, 18: 2343–2350. 10.1046/j.1460-9568.2003.02952.x

    Article  PubMed  Google Scholar 

  73. Gao YJ, Ren WH, Zhang YQ, Zhao ZQ: Contributions of the anterior cingulate cortex and amygdala to pain- and fear-conditioned place avoidance in rats. Pain 2004, 110: 343–353. 10.1016/j.pain.2004.04.030

    Article  PubMed  Google Scholar 

  74. Pedersen LH, Scheel-Kruger J, Blackburn-Munro G: Amygdala GABA-A receptor involvement in mediating sensory-discriminative and affective-motivational pain responses in a rat model of peripheral nerve injury. Pain 2007, 127: 17–26. 10.1016/j.pain.2006.06.036

    Article  CAS  PubMed  Google Scholar 

  75. Ansah OB, Bourbia N, Goncalves L, Almeida A, Pertovaara A: Influence of amygdaloid glutamatergic receptors on sensory and emotional pain-related behavior in the neuropathic rat. Behav Brain Res 2010, 209: 174–178. 10.1016/j.bbr.2010.01.021

    Article  CAS  PubMed  Google Scholar 

  76. Crock LW, Kolber BJ, Morgan CD, Sadler KE, Vogt SK, Bruchas MR, Gereau RW: Central amygdala metabotropic glutamate receptor 5 in the modulation of visceral pain. J Neurosci 2012, 32: 14217–14226. 10.1523/JNEUROSCI.1473-12.2012

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  77. Kolber BJ, Montana MC, Carrasquillo Y, Xu J, Heinemann SF, Muglia LJ, Gereau RW: Activation of metabotropic glutamate receptor 5 in the amygdala modulates pain-like behavior. J Neurosci 2010, 30: 8203–8213. 10.1523/JNEUROSCI.1216-10.2010

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  78. Han JS, Neugebauer V: mGluR1 and mGluR5 antagonists in the amygdala inhibit different components of audible and ultrasonic vocalizations in a model of arthritic pain. Pain 2005, 113: 211–222. 10.1016/j.pain.2004.10.022

    Article  CAS  PubMed  Google Scholar 

  79. Palazzo E, Fu Y, Ji G, Maione S, Neugebauer V: Group III mGluR7 and mGluR8 in the amygdala differentially modulate nocifensive and affective pain behaviors. Neuropharmacology 2008, 55: 537–545. 10.1016/j.neuropharm.2008.05.007

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  80. Ortiz JP, Close LN, Heinricher MM, Selden NR: Alpha(2)-noradrenergic antagonist administration into the central nucleus of the amygdala blocks stress-induced hypoalgesia in awake behaving rats. Neuroscience 2008, 157: 223–228. 10.1016/j.neuroscience.2008.08.051

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  81. Deyama S, Takishita A, Tanimoto S, Ide S, Nakagawa T, Satoh M, Minami M: Roles of beta- and alpha2-adrenoceptors within the central nucleus of the amygdala in the visceral pain-induced aversion in rats. J Pharmacol Sci 2010, 114: 123–126. 10.1254/jphs.10139SC

    Article  CAS  PubMed  Google Scholar 

  82. Han JS, Adwanikar H, Li Z, Ji G, Neugebauer V: Facilitation of synaptic transmission and pain responses by CGRP in the amygdala of normal rats. Mol Pain 2010, 6: 10. 10.1186/1744-8069-6-10

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  83. Han JS, Li W, Neugebauer V: Critical role of calcitonin gene-related peptide 1 receptors in the amygdala in synaptic plasticity and pain behavior. J Neurosci 2005, 25: 10717–10728. 10.1523/JNEUROSCI.4112-05.2005

    Article  CAS  PubMed  Google Scholar 

  84. Ji G, Fu Y, Adwanikar H, Neugebauer V: Non-pain-related CRF1 activation in the amygdala facilitates synaptic transmission and pain responses. Mol Pain 2013, 9: 2. 10.1186/1744-8069-9-2

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  85. Ji G, Fu Y, Ruppert KA, Neugebauer V: Pain-related anxiety-like behavior requires CRF1 receptors in the amygdala. Mol Pain 2007, 3: 13. 10.1186/1744-8069-3-13

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  86. Fu Y, Neugebauer V: Differential mechanisms of CRF1 and CRF2 receptor functions in the amygdala in pain-related synaptic facilitation and behavior. J Neurosci 2008, 28: 3861–3876. 10.1523/JNEUROSCI.0227-08.2008

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  87. Qin C, Greenwood-Van Meerveld B, Foreman RD: Spinal neuronal responses to urinary bladder stimulation in rats with corticosterone or aldosterone onto the amygdala. J Neurophysiol 2003, 90: 2180–2189. 10.1152/jn.00298.2003

    Article  CAS  PubMed  Google Scholar 

  88. Qin C, Greenwood-Van Meerveld B, Myers DA, Foreman RD: Corticosterone acts directly at the amygdala to alter spinal neuronal activity in response to colorectal distension. J Neurophysiol 2003, 89: 1343–1352.

    Article  CAS  PubMed  Google Scholar 

  89. Myers B, Dittmeyer K, Greenwood-Van Meerveld B: Involvement of amygdaloid corticosterone in altered visceral and somatic sensation. Behav Brain Res 2007, 181: 163–167. 10.1016/j.bbr.2007.03.031

    Article  CAS  PubMed  Google Scholar 

  90. Myers B, Greenwood-Van Meerveld B: Corticosteroid receptor-mediated mechanisms in the amygdala regulate anxiety and colonic sensitivity. Am J Physiol Gastrointest Liver Physiol 2007, 292: G1622–1629. 10.1152/ajpgi.00080.2007

    Article  CAS  PubMed  Google Scholar 

  91. Fu Y, Han J, Ishola T, Scerbo M, Adwanikar H, Ramsey C, Neugebauer V: PKA and ERK, but not PKC, in the amygdala contribute to pain-related synaptic plasticity and behavior. Mol Pain 2008, 4: 26. 10.1186/1744-8069-4-26

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  92. Crown ED, King TE, Meagher MW, Grau JW: Shock-induced hyperalgesia: III. Role of the bed nucleus of the stria terminalis and amygdaloid nuclei. Behav Neurosci 2000, 114: 561–573.

    Article  CAS  PubMed  Google Scholar 

  93. Rhudy JL, Meagher MW: Negative affect: effects on an evaluative measure of human pain. Pain 2003, 104: 617–626. 10.1016/S0304-3959(03)00119-2

    Article  PubMed  Google Scholar 

  94. Yalcin I, Bohren Y, Waltisperger E, Sage-Ciocca D, Yin JC, Freund-Mercier MJ, Barrot M: A time-dependent history of mood disorders in a murine model of neuropathic pain. Biol Psychiatry 2011, 70: 946–953. 10.1016/j.biopsych.2011.07.017

    Article  PubMed  Google Scholar 

  95. Calvino B, Besson JM, Boehrer A, Depaulis A: Ultrasonic vocalization (22–28 kHz) in a model of chronic pain, the arthritic rat: effects of analgesic drugs. NeuroReport 1996, 7: 581–584. 10.1097/00001756-199601310-00049

    Article  CAS  PubMed  Google Scholar 

  96. Kulkarni B, Bentley DE, Elliott R, Julyan PJ, Boger E, Watson A, Boyle Y, El-Deredy W, Jones AK: Arthritic pain is processed in brain areas concerned with emotions and fear. Arthritis Rheum 2007, 56: 1345–1354. 10.1002/art.22460

    Article  CAS  PubMed  Google Scholar 

  97. Petrovic P, Ingvar M, Stone-Elander S, Petersson KM, Hansson P: A PET activation study of dynamic mechanical allodynia in patients with mononeuropathy. Pain 1999, 83: 459–470. 10.1016/S0304-3959(99)00150-5

    Article  CAS  PubMed  Google Scholar 

  98. Yoshino A, Okamoto Y, Onoda K, Yoshimura S, Kunisato Y, Demoto Y, Okada G, Yamawaki S: Sadness enhances the experience of pain via neural activation in the anterior cingulate cortex and amygdala: an fMRI study. NeuroImage 2010, 50: 1194–1201. 10.1016/j.neuroimage.2009.11.079

    Article  PubMed  Google Scholar 

  99. Younger J, Aron A, Parke S, Chatterjee N, Mackey S: Viewing pictures of a romantic partner reduces experimental pain: involvement of neural reward systems. PLoS One 2010, 5: e13309. 10.1371/journal.pone.0013309

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  100. Delaney AJ, Crane JW, Sah P: Noradrenaline modulates transmission at a central synapse by a presynaptic mechanism. Neuron 2007, 56: 880–892. 10.1016/j.neuron.2007.10.022

    Article  CAS  PubMed  Google Scholar 

  101. Yamano M, Hillyard CJ, Girgis S, Emson PC, MacIntyre I, Tohyama M: Projection of neurotensin-like immunoreactive neurons from the lateral parabrachial area to the central amygdaloid nucleus of the rat with reference to the coexistence with calcitonin gene-related peptide. Exp Brain Res 1988, 71: 603–610. 10.1007/BF00248753

    Article  CAS  PubMed  Google Scholar 

  102. Salio C, Averill S, Priestley JV, Merighi A: Costorage of BDNF and neuropeptides within individual dense-core vesicles in central and peripheral neurons. Dev Neurobiol 2007, 67: 326–338. 10.1002/dneu.20358

    Article  CAS  PubMed  Google Scholar 

  103. Sun N, Cassel MD: Intrinsic GABAergic neurons in the rat central extended amygdala. J Comp Neurol 1993, 330: 381–404. 10.1002/cne.903300308

    Article  CAS  PubMed  Google Scholar 

  104. Ciocchi S, Herry C, Grenier F, Wolff SB, Letzkus JJ, Vlachos I, Ehrlich I, Sprengel R, Deisseroth K, Stadler MB, et al.: Encoding of conditioned fear in central amygdala inhibitory circuits. Nature 2010, 468: 277–282. 10.1038/nature09559

    Article  CAS  PubMed  Google Scholar 

  105. Haubensak W, Kunwar PS, Cai H, Ciocchi S, Wall NR, Ponnusamy R, Biag J, Dong HW, Deisseroth K, Callaway EM, et al.: Genetic dissection of an amygdala microcircuit that gates conditioned fear. Nature 2010, 468: 270–276. 10.1038/nature09553

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  106. Dong YL, Fukazawa Y, Wang W, Kamasawa N, Shigemoto R: Differential postsynaptic compartments in the laterocapsular division of the central nucleus of amygdala for afferents from the parabrachial nucleus and the basolateral nucleus in the rat. J Comp Neurol 2010, 518: 4771–4791. 10.1002/cne.22487

    Article  PubMed  Google Scholar 

  107. Li W, Neugebauer V: Differential roles of mGluR1 and mGluR5 in brief and prolonged nociceptive processing in central amygdala neurons. J Neurophysiol 2004, 91: 13–24.

    Article  CAS  PubMed  Google Scholar 

  108. Ren W, Neugebauer V: Pain-related increase of excitatory transmission and decrease of inhibitory transmission in the central nucleus of the amygdala are mediated by mGluR1. Mol Pain 2010, 6: 93. 10.1186/1744-8069-6-93

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  109. Han JS, Bird GC, Neugebauer V: Enhanced group III mGluR-mediated inhibition of pain-related synaptic plasticity in the amygdala. Neuropharmacology 2004, 46: 918–926. 10.1016/j.neuropharm.2004.01.006

    Article  CAS  PubMed  Google Scholar 

  110. Han JS, Fu Y, Bird GC, Neugebauer V: Enhanced group II mGluR-mediated inhibition of pain-related synaptic plasticity in the amygdala. Mol Pain 2006, 2: 18. 10.1186/1744-8069-2-18

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  111. Li W, Neugebauer V: Differential changes of group II and group III mGluR function in central amygdala neurons in a model of arthritic pain. J Neurophysiol 2006, 96: 1803–1815. 10.1152/jn.00495.2006

    Article  CAS  PubMed  Google Scholar 

  112. Li Z, Ji G, Neugebauer V: Mitochondrial reactive oxygen species are activated by mGluR5 through IP3 and activate ERK and PKA to increase excitability of amygdala neurons and pain behavior. J Neurosci 2011, 31: 1114–1127. 10.1523/JNEUROSCI.5387-10.2011

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  113. Li W, Neugebauer V: Block of NMDA and non-NMDA receptor activation results in reduced background and evoked activity of central amygdala neurons in a model of arthritic pain. Pain 2004, 110: 112–122. 10.1016/j.pain.2004.03.015

    Article  CAS  PubMed  Google Scholar 

  114. Jaferi A, Pickel VM: Mu-opioid and corticotropin-releasing-factor receptors show largely postsynaptic co-expression, and separate presynaptic distributions, in the mouse central amygdala and bed nucleus of the stria terminalis. Neuroscience 2009, 159: 526–539. 10.1016/j.neuroscience.2008.12.061

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  115. Zhu W, Pan ZZ: Mu-opioid-mediated inhibition of glutamate synaptic transmission in rat central amygdala neurons. Neuroscience 2005, 133: 97–103. 10.1016/j.neuroscience.2005.02.004

    Article  CAS  PubMed  Google Scholar 

  116. Chieng BC, Christie MJ, Osborne PB: Characterization of neurons in the rat central nucleus of the amygdala: cellular physiology, morphology, and opioid sensitivity. J Comp Neurol 2006, 497: 910–927. 10.1002/cne.21025

    Article  CAS  PubMed  Google Scholar 

  117. Narita M, Kaneko C, Miyoshi K, Nagumo Y, Kuzumaki N, Nakajima M, Nanjo K, Matsuzawa K, Yamazaki M, Suzuki T: Chronic pain induces anxiety with concomitant changes in opioidergic function in the amygdala. Neuropsychopharmacology 2006, 31: 739–750. 10.1038/sj.npp.1300858

    Article  CAS  PubMed  Google Scholar 

  118. Finnegan TF, Chen SR, Pan HL: Effect of the mu opioid on excitatory and inhibitory synaptic inputs to periaqueductal gray-projecting neurons in the amygdala. J Pharmacol Exp Ther 2005, 312: 441–448.

    Article  CAS  PubMed  Google Scholar 

  119. Deyama S, Nakagawa T, Kaneko S, Uehara T, Minami M: Involvement of the bed nucleus of the stria terminalis in the negative affective component of visceral and somatic pain in rats. Behav Brain Res 2007, 176: 367–371. 10.1016/j.bbr.2006.10.021

    Article  PubMed  Google Scholar 

  120. Deyama S, Katayama T, Kondoh N, Nakagawa T, Kaneko S, Yamaguchi T, Yoshioka M, Minami M: Role of enhanced noradrenergic transmission within the ventral bed nucleus of the stria terminalis in visceral pain-induced aversion in rats. Behav Brain Res 2009, 197: 279–283. 10.1016/j.bbr.2008.08.024

    Article  CAS  PubMed  Google Scholar 

  121. Deyama S, Katayama T, Ohno A, Nakagawa T, Kaneko S, Yamaguchi T, Yoshioka M, Minami M: Activation of the beta-adrenoceptor-protein kinase A signaling pathway within the ventral bed nucleus of the stria terminalis mediates the negative affective component of pain in rats. J Neurosci 2008, 28: 7728–7736. 10.1523/JNEUROSCI.1480-08.2008

    Article  CAS  PubMed  Google Scholar 

  122. Deyama S, Ide S, Kondoh N, Yamaguchi T, Yoshioka M, Minami M: Inhibition of noradrenaline release by clonidine in the ventral bed nucleus of the stria terminalis attenuates pain-induced aversion in rats. Neuropharmacology 2011, 61: 156–160. 10.1016/j.neuropharm.2011.03.023

    Article  CAS  PubMed  Google Scholar 

  123. Ide S, Hara T, Ohno A, Tamano R, Koseki K, Naka T, Maruyama C, Kaneda K, Yoshioka M, Minami M: Opposing roles of corticotropin-releasing factor and neuropeptide Y within the dorsolateral bed nucleus of the stria terminalis in the negative affective component of pain in rats. J Neurosci 2013, 33: 5881–5894. 10.1523/JNEUROSCI.4278-12.2013

    Article  CAS  PubMed  Google Scholar 

  124. Connell K, Bolton N, Olsen D, Piomelli D, Hohmann AG: Role of the basolateral nucleus of the amygdala in endocannabinoid-mediated stress-induced analgesia. Neurosci Lett 2006, 397: 180–184. 10.1016/j.neulet.2005.12.008

    Article  CAS  PubMed  Google Scholar 

  125. Rea K, Olango WM, Harhen B, Kerr DM, Galligan R, Fitzgerald S, Moore M, Roche M, Finn DP: Evidence for a role of GABAergic and glutamatergic signalling in the basolateral amygdala in endocannabinoid-mediated fear-conditioned analgesia in rats. Pain 2013, 154: 576–585. 10.1016/j.pain.2012.12.021

    Article  CAS  PubMed  Google Scholar 

  126. Rea K, Roche M, Finn DP: Modulation of conditioned fear, fear-conditioned analgesia, and brain regional c-Fos expression following administration of muscimol into the rat basolateral amygdala. J Pain 2011, 12: 712–721. 10.1016/j.jpain.2010.12.010

    Article  CAS  PubMed  Google Scholar 

  127. Helmstetter FJ: Stress-induced hypoalgesia and defensive freezing are attenuated by application of diazepam to the amygdala. Pharmacol Biochem Behav 1993, 44: 433–438. 10.1016/0091-3057(93)90487-E

    Article  CAS  PubMed  Google Scholar 

  128. Nandigama P, Borszcz GS: Affective analgesia following the administration of morphine into the amygdala of rats. Brain Res 2003, 959: 343–354. 10.1016/S0006-8993(02)03884-2

    Article  CAS  PubMed  Google Scholar 

  129. Helmstetter FJ, Bellgowan PS, Poore LH: Microinfusion of mu but not delta or kappa opioid agonists into the basolateral amygdala results in inhibition of the tail flick reflex in pentobarbital-anesthetized rats. J Pharmacol Exp Ther 1995, 275: 381–388.

    CAS  PubMed  Google Scholar 

  130. McGaraughty S, Heinricher MM: Microinjection of morphine into various amygdaloid nuclei differentially affects nociceptive responsiveness and RVM neuronal activity. Pain 2002, 96: 153–162. 10.1016/S0304-3959(01)00440-7

    Article  CAS  PubMed  Google Scholar 

  131. Chen J, Song Y, Yang J, Zhang Y, Zhao P, Zhu XJ, Su HC: The contribution of TNF-alpha in the amygdala to anxiety in mice with persistent inflammatory pain. Neurosci Lett 2013, 541: 275–280.

    Article  CAS  PubMed  Google Scholar 

  132. Ji G, Sun H, Fu Y, Li Z, Pais-Vieira M, Galhardo V, Neugebauer V: Cognitive impairment in pain through amygdala-driven prefrontal cortical deactivation. J Neurosci 2010, 30: 5451–5464. 10.1523/JNEUROSCI.0225-10.2010

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  133. Deyama S, Yamamoto J, Machida T, Tanimoto S, Nakagawa T, Kaneko S, Satoh M, Minami M: Inhibition of glutamatergic transmission by morphine in the basolateral amygdaloid nucleus reduces pain-induced aversion. Neurosci Res 2007, 59: 199–204. 10.1016/j.neures.2007.06.1473

    Article  CAS  PubMed  Google Scholar 

  134. Watabe AM, Ochiai T, Nagase M, Takahashi Y, Sato M, Kato F: Synaptic potentiation in the nociceptive amygdala following fear learning in mice. Mol Brain 2013, 6: 11. 10.1186/1756-6606-6-11

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by Centre National de la Recherche Scientifique (UPR3212) and University of Strasbourg.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Pierre Veinante.

Additional information

Competing interests

Dr. Barrot received lecture fees from Adir and Lilly France, and contract from Missions-Cadres. Drs. Barrot and Yalcin reported a CNRS-filed patent for pain treatments. Other authors reported no potential conflicts of interest.

Authors’ contributions

PV conceived this review; PV, IY and MB wrote and revised the manuscript. All authors read and approved the final manuscript.

Authors’ original submitted files for images

Below are the links to the authors’ original submitted files for images.

Authors’ original file for figure 1

Authors’ original file for figure 2

Authors’ original file for figure 3

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Veinante, P., Yalcin, I. & Barrot, M. The amygdala between sensation and affect: a role in pain. J Mol Psychiatr 1, 9 (2013). https://doi.org/10.1186/2049-9256-1-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/2049-9256-1-9

Keywords